One-step triplex PCR/RT-PCR to detect canine distemper virus, canine parvovirus, and canine kobuvirus.

نویسندگان

  • Dafei Liu
  • Fei Liu
  • Dongchun Guo
  • Xiaoliang Hu
  • Zhijie Li
  • Zhigang Li
  • Jianzhang Ma
  • Chunguo Liu
چکیده

To rapidly distinguish Canine distemper virus (CDV), canine parvovirus (CPV), and canine kobuvirus (CaKoV) in practice, a one-step multiplex PCR/RT-PCR assay was developed, with detection limits of 102.1 TCID50 for CDV, 101.9 TCID50 for CPV and 103 copies for CaKoV. This method did not amplify nonspecific DNA or RNA from other canine viruses. Therefore, the assay provides a sensitive tool for the rapid clinical detection and epidemiological surveillance of CDV, CPV and CaKoV in dogs.

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عنوان ژورنال:
  • The Journal of veterinary medical science

دوره   شماره 

صفحات  -

تاریخ انتشار 2018